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CERTIFICATE OF ANALYSIS

| PRODUCT: Bleomycin Sulfate, Streptomyces verticillus. (mixture of Bleomycin A2, B2 & B4) |
PRODUCT No.: B526 |
| CAS: 9041-93-4 | LOT No.: B-N210401 |
| MW: 1415.55 (for A2) & 1425.51 (for B2) | STORE: +4º C, Protect from Light |
| RTECS: EC5991990 | TOXICITY: Hazardous Highly Toxic if swallowed: May cause heritable genetic damage. |
| FORMULA: C55H84N17O21S3 (for A2) C55H84N20O21S2 (for B2) |
| Analysis | Specification (USP 31) | Results |
|---|---|---|
| Appearance | White to off-white powder | Conforms |
| Identification | Conforms to Standard | Conforms |
| Bleomycin Contents: A2 B2 B4 |
. ≥55 – 70% 25 – 32% ≤1% |
. 67% 30% 0.05% |
| Purity (HPLC) | ≥90% (A2 + B2) | 97% |
| Copper content | ≤0.1% | 0.0% |
| L.O.D. | ≤6.0% | 2.9% |
| Potency (USP Bleomycin Units) | 1.5 – 2.0 U/mg | 1.7 U/mg |
| PH | 4.5 – 6.0 | 5 |
| Solubility | Freely Soluble in H2O (20mg/mL) | Conforms |
| Stability | Stable at +4º C if desiccated and properly stored for at least 3 years. Stock solution stable for a month at +4º C. Ships ambient. |
| QUALITY ASSURANCE: | Verified upon data received from manufacturer. |
FOR RESEARCH or FURTHER PROCESSING – NOT FOR HUMAN or DRUG USE
